HiTrap IXSelect: pour la purification du facteur de coagulation IX. HiTrap IgY: designed for purification of IgY. HiTrap IgM: designed for purification of monoclonal IgM. HiTrap Capto adhere is a multimodal anion exchanger. HiTrap MabSelect™, MabSelect SuRe™ and MabSelect Xtra™: developed to meet the productivity and sanitisation requirements of industry to develop large-scale antibody purification procedures. Q (anion exchange), S or SP (cation exchange) are fully charged over a broad pH range. GE, the GE Monogram, Capto, HiTrap, Mono Q, Mono S, MonoBeads, and. Capto Q has a strong quaternary ammonium (Q) anion exchanger coupled to a chemically modified, high-flow agarose matrix. New Capto HiRes prepacked columns can separate biomolecules with small differences. HiTrap Protein A and HiTrap Protein G: designed for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants. HiTrap Capto Q is a strong anion exchanger prepacked with BioProcess Capto media for screening, and small-scale protein purifications using ion exchange chromatography (IEX). HiTrap NHS: high-performance medium designed for easy coupling of ligands containing primary amines. HiTrap Capto™: designed to meet industrial requirements for the production of biological molecules. HiTrap Capto Q, Capto S and Capto DEAE columns provide fast, reproducible and easy separations in a convenient format. It is possible to connect columns to each other for achieving columns of variable volume from 1 to 20 ml as requiredĪcronyms and abbreviations: Q = Quaternary ammonium / DEAE = Diethylaminoethyle / CM = Carboxymethyle / S = Methylsulfonate / ANX = Diethylaminopropyle Each HiTrap™ column is equiped with one 1/16" male luer and one 1/16" female luer.HiTrap columns are available in 1 ml and 5 ml bed volume.
#Hitrap capto q pdf manual#
HiTrap IgM: designed for purification of monoclonal IgM. system that allows you to automate manual protein purification procedures in academic and educational labs. HiTrap MabSelect™, MabSelect SuRe™ and MabSelect Xtra™: developed to meet the productivity and sanitisation requirements of industry to develop large-scale antibody purification procedures. HiTrap Protein A and HiTrap Protein G: designed for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants. Purification of immunoglobulins Acronyms and abbreviations: Q = Quaternary ammonium / DEAE = Diethylaminoethyle / CM = Carboxymethyle / S = Methylsulfonate / ANX = Diethylaminopropyle HiTrap Capto™: designed to meet industrial requirements for the production of biological molecules.It is possible to connect columns to each other for achieving columns of variable volume from 1 to 20 ml as required R e q u ir e s a s yr in g e o r p ump to u n. Dynamic binding capacity, Q B10 80 mg human IgG/mL resin at 6 min residence time 65 mg human IgG /mL resin at 4 min residence time Recommended maximum operating flow velocity 300 cm/h pH stability, operational 312 pH stability, CIP 214 Chemical stability Stable to commonly used aqueous buffers in Protein A chromatography. HiT ra p i D e sa lt n g 1 7-1408-0 1 0.25 to 1.5 m l 1.0 to 2.0 m l P rep a cked w it h Se p h adex G-25 Su p e rf in e. AAV5 polishing step with Capto Q ImpRes resin to 51 mL HiScale column is. For desal ting and buffe r e xch a n g e o f p ro te in extracts (M > r 5 000). raphy with the Capto AVB, HiTrap col- umn. Each HiTrap™ column is equiped with one 1/16" male luer and one 1/16" female luer. q u ir e s a la b o ra to ry p ump o ra chro matography system to run.
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